© Fifth Third Bancorp | All Rights Reserved © Fifth Third Bancorp | All Rights Reserved Morgan Stanley US Financials, Payments, & CRE Conference Tim Spence President and Chief Executive Officer June 14, 2023

© Fifth Third Bancorp | All Rights Reserved This presentation contains statements that we believe are “forward-looking statements” within the meaning of Section 27A of the Securities Act of 1933, as amended, and Rule 175 promulgated thereunder, and Section 21E of the Securities Exchange Act of 1934, as amended, and Rule 3b-6 promulgated thereunder. All statements other than statements of historical fact are forward-looking statements. These statements relate to our financial condition, results of operations, plans, objectives, future performance, capital actions or business. They usually can be identified by the use of forward-looking language such as “will likely result,” “may,” “are expected to,” “is anticipated,” “potential,” “estimate,” “forecast,” “projected,” “intends to,” or may include other similar words or phrases such as “believes,” “plans,” “trend,” “objective,” “continue,” “remain,” or similar expressions, or future or conditional verbs such as “will,” “would,” “should,” “could,” “might,” “can,” or similar verbs. You should not place undue reliance on these statements, as they are subject to risks and uncertainties, including but not limited to the risk factors set forth in our most recent Annual Report on Form 10-K as updated by our filings with the U.S. Securities and Exchange Commission (“SEC”). There are a number of important factors that could cause future results to differ materially from historical performance and these forward-looking statements. Factors that might cause such a difference include, but are not limited to: (1) effects of the global COVID-19 pandemic; (2) deteriorating credit quality; (3) loan concentration by location or industry of borrowers or collateral; (4) problems encountered by other financial institutions; (5) inadequate sources of funding or liquidity; (6) unfavorable actions of rating agencies; (7) inability to maintain or grow deposits; (8) limitations on the ability to receive dividends from subsidiaries; (9) cyber-security risks; (10) Fifth Third’s ability to secure confidential information and deliver products and services through the use of computer systems and telecommunications networks; (11) failures by third-party service providers; (12) inability to manage strategic initiatives and/or organizational changes; (13) inability to implement technology system enhancements; (14) failure of internal controls and other risk management systems; (15) losses related to fraud, theft, misappropriation or violence; (16) inability to attract and retain skilled personnel; (17) adverse impacts of government regulation; (18) governmental or regulatory changes or other actions; (19) failures to meet applicable capital requirements; (20) regulatory objections to Fifth Third’s capital plan; (21) regulation of Fifth Third’s derivatives activities; (22) deposit insurance premiums; (23) assessments for the orderly liquidation fund; (24) replacement of LIBOR; (25) weakness in the national or local economies; (26) global political and economic uncertainty or negative actions; (27) changes in interest rates and the effects of inflation; (28) changes and trends in capital markets; (29) fluctuation of Fifth Third’s stock price; (30) volatility in mortgage banking revenue; (31) litigation, investigations, and enforcement proceedings by governmental authorities; (32) breaches of contractual covenants, representations and warranties; (33) competition and changes in the financial services industry; (34) changing retail distribution strategies, customer preferences and behavior; (35) difficulties in identifying, acquiring or integrating suitable strategic partnerships, investments or acquisitions; (36) potential dilution from future acquisitions; (37) loss of income and/or difficulties encountered in the sale and separation of businesses, investments or other assets; (38) results of investments or acquired entities; (39) changes in accounting standards or interpretation or declines in the value of Fifth Third’s goodwill or other intangible assets; (40) inaccuracies or other failures from the use of models; (41) effects of critical accounting policies and judgments or the use of inaccurate estimates; (42) weather-related events, other natural disasters, or health emergencies (including pandemics); (43) the impact of reputational risk created by these or other developments on such matters as business generation and retention, funding and liquidity; (44) changes in law or requirements imposed by Fifth Third’s regulators impacting our capital actions, including dividend payments and stock repurchases; and (45) Fifth Third's ability to meet its environmental and/or social targets, goals and commitments. You should refer to our periodic and current reports filed with the Securities and Exchange Commission, or “SEC,” for further information on other factors, which could cause actual results to be significantly different from those expressed or implied by these forward-looking statements. Moreover, you should treat these statements as speaking only as of the date they are made and based only on information then actually known to us. We expressly disclaim any obligation or undertaking to release publicly any updates or revisions to any forward-looking statements contained herein to reflect any change in our expectations or any changes in events, conditions or circumstances on which any such statement is based, except as may be required by law, and we claim the protection of the safe harbor for forward-looking statements contained in the Private Securities Litigation Reform Act of 1995. The information contained herein is intended to be reviewed in its totality, and any stipulations, conditions or provisos that apply to a given piece of information in one part of this press release should be read as applying mutatis mutandis to every other instance of such information appearing herein. Copies of those filings are available at no cost on the SEC’s website at www.sec.gov or on our website at www.53.com. Annualized, pro forma, projected and estimated numbers are used for illustrative purpose only, are not forecasts and may not reflect actual results. In this presentation, we may sometimes provide non-GAAP financial information. Please note that although non-GAAP financial measures provide useful insight to analysts, investors and regulators, they should not be considered in isolation or relied upon as a substitute for analysis using GAAP measures. We provide a discussion of non-GAAP measures and reconciliations to the most directly comparable GAAP measures in later slides in this presentation, as well as on pages 26 through 28 of our 1Q23 earnings release. Management does not provide a reconciliation for forward-looking non-GAAP financial measures where it is unable to provide a meaningful or accurate calculation or estimation of reconciling items and the information is not available without unreasonable effort. This is due to the inherent difficulty of forecasting the occurrence and the financial impact of various items that have not yet occurred, are out of the Bancorp's control or cannot be reasonably predicted. For the same reasons, Bancorp's management is unable to address the probable significance of the unavailable information. Forward-looking non-GAAP financial measures provided without the most directly comparable GAAP financial measures may vary materially from the corresponding GAAP financial measures. This presentation incorporates the following peers: CFG, CMA, FCNCA (where applicable), FHN, HBAN, KEY, MTB, PNC, RF, TFC, USB, & ZION. Cautionary statement 2

© Fifth Third Bancorp | All Rights Reserved Why Fifth Third Continually investing for long-term outperformance regardless of the environment Through the cycle discipline throughout the bank – liquidity/deposits, credit, rates, expenses, and capital management Focused on consistently generating top quartile profitability among peers Stability Profitability Growth Consistent and disciplined management, with a long-term focus throughout the company 3 Positioned to perform the best in the most difficult times

© Fifth Third Bancorp | All Rights Reserved We have grown our high-quality deposit franchise through multi- year investments 4 1 Commercial segment service charges on deposits as a % of noninterest revenue; 2 See forward-looking statements on page 2 of this presentation regarding forward-looking non-GAAP measures Significantly better product offerings less focused on rate are helping improve the noninterest-bearing mix of deposits 21% 32% Noninterest-bearing deposits as a % of total core deposits Treasury management fees 1 As a % of noninterest revenue 6% 13% Expect investments throughout deposit franchise to result in durable competitive advantages and improved noninterest-bearing mix compared to last time the Fed funds was at 5.25% 2 1Q23 5.25% Fed funds Consumer noninterest-bearing checking accounts As a % of total consumer checking accounts ~60% ~85% 2Q06 5.25% Fed funds Select investments • Award-winning geospatial capabilities leveraged for selecting branch locations • 75+ AI/ML models running proprietary MyDay portal / customer recommendation engine, informing direct marketing campaigns, and other deposit initiatives • Analytically driven market research informing new product development • Momentum checking with best-in-class features and a continuous improvement philosophy • Peer leading Treasury Management with balance among legacy, managed services, and embedded payments • Consistent investments in high-growth, mid-sized metro markets primarily in the Southeast • Added 80+ branches since 2017 (#2 among U.S. banks within footprint) • Customer-centric “NextGen” branch design Branch network Products Data driven insights

© Fifth Third Bancorp | All Rights Reserved 0.00% 0.10% 0.20% 0.30% 0.40% 0.50% 0.60% 0.00% 0.10% 0.20% 0.30% 0.40% 0.50% 0.60% Our deposit franchise stands out favorably among peers 5 Only bank among peers to increase deposits since 2Q22 Note: Banks impacted by M&A excluded from analysis ACH Credit receive / Consumer Deposits A C H D e b it s e n d / C o m m e r c i a l D e p o s it s 2022 ACH data from NACHA; deposit balances as of 4Q22 High percentage of operating deposit relationships in both commercial and consumer Period-end total deposits; indexed to 100 85 90 95 100 105 2Q22 3Q22 4Q22 1Q23 +1% cumulative increase Fed H.8 (4%) cumulative decrease Peer median (6%) cumulative decrease

© Fifth Third Bancorp | All Rights Reserved 6 Presence 1 Data sourced from S&P Global Market Intelligence with deposits per branch capped at $250MM per June 2022 FDIC data; Total and Midwest rankings represent deposit market share and Southeast ranking is based on locational share; 2 See forward-looking statements on page 2 of this presentation regarding forward-looking non-GAAP measures Consistent and focused investments in the Consumer deposit franchise Products Consistent focus has led to 3% annualized organic household growth over the past 5 years • 90%+ of branches added since 2017 are in the Southeast • NextGen design in >95% of new builds #2 in FITB Midwest MSAs 1 #6 in FITB Southeast MSAs 1 • ~1.2 million Fifth Third Momentum households (~50% of total consumer) • Not just a front book product • Noninterest bearing checking product, given the other valuable services provided • Higher primacy and higher retention than previous new-to-bank customers • Median age of 36, and a ~$9,000 avg. deposit balance • Leveraging AI/ML capabilities to personalize 100+ recommendations • Gives retail bankers 20-25 personalized, incentive-based offers to support strategic objectives with increased effectiveness • ~1 billion targeted annual engagement opportunities #4 in deposit share 1 Significant market share gains in high-growth Southeast MSAs of focus 1 #1 excluding banks >$1T Fifth Third Momentum Banking Retail Banker MyDay dashboard helping improve productivity ~25%: Example: Momentum Banking Example: Customer recommendation engine Process Locational share rank Sarasota, FL Jacksonville, FL Raleigh-Durham, NC Orlando, FL Greenville, SC Nashville, TN Charlotte, NC Tampa, FL #5 #9 #15 #7 #31 #6 #5 #6 #5 #8 #8 #7 #25 #3 #4 #6 #5 #7 #4 #6 #10 #2 #4 #6 2018 2022 2025E 2

© Fifth Third Bancorp | All Rights Reserved Led by Expert AP, Expert AR, and Cash Logistics • Digitize and automate manual “order-to-cash” and “procure-to-pay” processes for clients • Expect mid-to-high single digits annual growth, reflecting acquisition of BD Healthcare Commercial deposit franchise led by peer-leading treasury management business 7 Commercial deposit franchise highlights 1.3% 1.9% 2.0% 2.4% 2.5% 2.5% 2.5% 2.6% 3.8% 4.3% 4.5% 4.5% 5.0% Peer 12 Peer 11 Peer 10 Peer 9 Peer 8 Peer 7 Peer 6 Peer 5 Peer 4 Peer 3 Peer 2 Peer 1 x Total deposit fees less consumer (OD, maintenance, and ATM fees) relative to total adjusted revenue; 1Q23 LTM 1 1 Source: Company filings; 2 Insured by FDIC product type; 3 Source: Rankings are based on data provided by survey participants in the 2022 Cash Management Services Survey administered by EY; 4 Represents forward looking statement, please refer to page 2 of this presentation regarding forward-looking non-GAAP measures #2 of 38 in Coin and currency revenue #2 of 33 in Retail lockbox remittances #4 of 43 in Total check clearing #3 of 45 in Total ACH originations #5 of 35 in Account reconciliations #4 of 39 in Wholesale lockbox remittances #8 of 30 in Controlled disbursement #6 of 30 in Purchasing cards #10 of 33 in Demand deposit accounts Peer-leading Treasury Management revenue performance Expect balanced future state TM business revenue contributions 4 Legacy TM Managed Services Embedded Payments • 88% of commercial balances represented by relationships that utilize Treasury Management services (including 73% of uninsured) • Balanced-weighted relationship age of 24 years • Median relationship deposit balance of $375K • 26% FDIC insured 2 • Launched Newline TM to accelerate embedded payments capabilities which will add to commercial deposit growth Top 10 Ranking in 2022 EY Cash Management Survey 3 Launch of Newline TM to accelerate embedded payments business • Expect growth to accelerate in 2024 and beyond, reflecting acquisition of Rize Money Expect legacy TM growth to be consistent with nominal GDP

© Fifth Third Bancorp | All Rights Reserved Rize acquisition accelerates long standing embedded payments capabilities 8 Combining over a decade of embedded payments expertise with acquisition of Rize Money to form Newline TM by Fifth Third Client list includes a broad range of category leaders • Expect FY23 revenue of ~$130MM (incorporated into April 2023 guide) • Expect FY23 deposit growth of ~40% (expect 4Q23 balances of ~$2B) Acquirer BIN (bank identification number) sponsorship Issuing BIN sponsorship Credit Card BIN sponsorship Originating depository financial institution ACH / Wire processing Deposit Account Solutions Embedded payments at-a-glance 1 : • Modern, innovative, and highly-scalable embedded payments product platform • Large scale suite of payment types (debit, credit, ACH, RTP, wire, FedNow, etc.) • Allows clients to customize 20 APIs with functionality for 70+ product features • Differentiated capabilities with a focus on AML/BSA compliance; not just BaaS 2009 Post FTPS (Vantiv) spinoff Rize Money acquisition (2023) Launch of Newline TM (2023) to deliver innovative solutions and further accelerate growth of new quality relationships Payroll Consumer Payments Business Payments 1 Represents forward looking statement, please refer to page 2 of this presentation regarding forward-looking non-GAAP measures RTP 2019 Post MB Financial acquisition 2022 2019 2023E Total Revenue Largest domestic payroll provider Issuing relationship with one of the top 3 core providers in the US Primary relationship with top 3 domestic provider and 2 of top 4 non-bank acquirers Merchant acquiring Relationship with leading SMB human capital management firm Relationship with leading small business payment provider

© Fifth Third Bancorp | All Rights Reserved 9 Conservative consumer loan portfolio highly concentrated in super prime borrowers and homeowners 1 April 2023 vs. April 2020 Additional information: ~20% ~(20%) Homeowners Renters ~25% ~(5%) FICO 720+ FICO <720 Median FITB customer deposit balance compared to 3 years ago 1 • ~85% of total consumer portfolio balances represented by homeowners (U.S. homeownership rate of ~66%) • ~75% of Auto and Card balances represented by homeowners • Thesis: consumers who largely locked in historically low fixed-rate mortgage payments will be better able to offset inflationary pressures Homeowners Prime and Super Prime Focus • Total consumer WA FICO at origination of 765 • ~80% of Consumer portfolio has a 720+ FICO • Minimized impact of stimulus-related FICO score warping (0 to 20 points; average 5) by underwriting to trended scores, using internal models, and tightening underwriting standards 63% 16% 18% 3% 750+ 720-749 660-719 <660 Positive selection characteristics Total Consumer portfolio FICO score at origination ~85% ~15% Homeowners Renters Total Consumer portfolio as of 1Q23

© Fifth Third Bancorp | All Rights Reserved 15% 14% 13% 8%8% 7% 6% 6% 4% 5% 4% 10% Real Estate Manufacturing Financial Services & Insurance Business Services Health Care Wholesale Trade Retail Trade Accommodation & Food Mining Communication & Information Construction Other 13% 14% 14% 15% 16% 16% 17% 21% 22% 30% 33% 36% 41% x Peer 1 Peer 2 Peer 3 Peer 4 Peer 5 Peer 6 Peer 7 Peer 8 Peer 9 Peer 10 Peer 11 Peer 12 10 Disciplined client selection throughout commercial Have maintained lowest CRE concentration among peers; office had already been de-emphasized for several years Disciplined client selection and well-diversified commercial portfolio to achieve strong credit results through the cycle 1 Source: Regulatory filings • Underwriting standards have been tightened since early 2020 with lower exceptions; 90% of total commercial portfolio has been underwritten (or re-underwritten) since Jan 2020 • Credits stressed on a +200 bps rate scenario vs. the forward curve (was +100 bps in 2021) • Focused corporate banking coverage on targeted industry verticals with specialized coverage, underwriting, and risk support • Criticized asset ratio stable over past several quarters • ~70% of mortgage CRE is amortizing Total Bancorp loans $124BN • Office CRE of $1.6B represents 1.3% of total loans • LTV range of 55 – 60% at origination; focus on disciplined regional and national clients with longstanding relationships • Average commit of $9.5MM; conservative underwriting limiting amount of credit extended • 0.01% delinquency rate; net recovery of (0.01%) over the LTM ended 1Q23 • Currently not pursuing new Office CRE originations CRE as a % of total loans 1 Well diversified commercial portfolio $77B Commercial portfolio balances by NAICS code Minimal non-owner-occupied office CRE with strong credit quality

© Fifth Third Bancorp | All Rights Reserved Net interest income 1 Noninterest income 1 up 2 – 3% Noninterest expense 1 down 8 – 9% down ~1% (1Q23 baseline: $1.522BN) (1Q23 baseline: $723MM) (1Q23 baseline: $1.319BN) Net charge-off ratio 25 – 35 bps 1 Non-GAAP measure: see forward-looking statements on page 2 of this presentation regarding forward-looking non-GAAP measures and use of non-GAAP measures on pages 26-28 of the 1Q23 earnings release. Please see cautionary statements on page 2 Total revenue 1 stable (1Q23 baseline: $2.249BN; Includes securities g/l) (including HFS) Avg. loans & leases stable to up 1% Current expectations 2Q23 compared to 1Q23 11 assumes a 25 basis point rate hike in May 2023 Allowance for credit losses expect ~$100MM build As of 4/20/23 As of 6/13/23 up 2 – 3% down 8 – 9% down 4 – 5% 25 – 35 bps down 2 – 3% stable assumes stable Fed funds through the remainder of the quarter expect ~$100MM build Assuming no change to Moody’s economic scenario Avg. total deposits stable to up 1% stable Expect FY23 NII growth of 3 – 5% (FY22 baseline: $5.625BN)

© Fifth Third Bancorp | All Rights Reserved 12 Appendix

© Fifth Third Bancorp | All Rights Reserved Classification: Internal Use 13 Addressing climate change Promoting inclusion and diversity Demonstrating our commitment to employees Strengthening our communities Keeping the customer at the center $165MM in lending, investments, and philanthropy towards Empowering Black Futures Neighborhood Investment Program 3 $1.4BN provided in community development lending and investment in 2022 ~$39MM in charitable donations to support communities ~118K hours of community service “Outstanding” rating on most recent Community Reinvestment Act performance examination from the Office of the Comptroller of Currency $20/hour minimum wage with over 40% of workforce receiving mid-year compensation increase Up to 7% 401(k) employer contribution with 80% participation ~776K hours of training (40 hours average / FTE) Decrease in overall turnover from 21.2% in 2021, to 21.0% in 2022 New flexible PTO policy including volunteer paid time away for full-time (8 hours) and part-time (4 hours) employees 12MM customer outreach calls, continuing our heightened connection to the customer 3.1% YoY consumer household growth compared to 1Q22 Low reliance on punitive consumer fees, with $13MM in NSF fees eliminated and $39MM in overdraft fees avoided with Extra Time ® $27BN deposited up to 2 days early with Early Pay® $26MM in consumer cash back rewards with 5/3 Cash/Back cards $2.9BN in lending, investments, financial accessibility and philanthropy towards $2.8BN AREEI initiative 1,2 36% board diversity 5 58% women; 28% persons of color in workforce >99% pay equity for women and minorities 8 $120MM Tier 1 diverse supplier spend, 11% of net addressable spend ~1K members in employee Sustainability Business Resource Group as of May 2023 ~$30BN in sustainable financing towards $100BN goal 1 285 due diligence reviews for sensitive sectors in compliance with E&S Risk Management Framework 4 50% reduction in Scope 1 and 2 GHG emissions since 2014 100% renewable energy purchased since 2019 Achieved carbon neutrality in our operations since 2020 6 $500MM inaugural Green Bond issued in October 2021 7 Fifth Third is committed to supporting customers, communities and employees Data is for fiscal year 2022, unless otherwise noted; 1 Data is through 3/31/2023; 2 Three-year $2.8BN commitment to Accelerate Racial Equity, Equality and Inclusion initiative timeframe is from 1/1/21 – 12/31/23; 3 Data is through 4/30/2023; Three-year $180MM Empowering Black Futures Neighborhood Investment Program is from 6/1/21 – 5/31/24; 4 9/23/2020 - 3/31/2022. The Environmental and Social Risk Management Framework (previously Environmental and Social Policy) can be found at ir.53.com/esg/environment; 5 Data is as of 4/18/23, in terms of ethnicity or gender; 6 For Scope 1, Scope 2 and business travel under Scope 3 emissions. Final CO2e emissions will be made available in 2023 following independent verification; 7 Sustainable Bond Report can be found at ir.53.com/esg/Sustainable-Bonds; 8 Refer to the 2021 ESG report for additional details on methodology. Sustainability priorities and metrics