NASDAQ: SNTI | sentibio.com SENTI-202: Positive Preliminary Results in the Treatment of Relapsed/Refractory Hematologic Malignancies Including AML in Ongoing Phase 1 Trial (SENTI-202-101) April 28, 2025 Conference Call and Webcast

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Company Highlights 3 • Demonstrated positive preliminary efficacy data in ongoing Phase 1 trial for treatment in R/R AML • Dose finding completed, CR* and durability data presented at AACR 2025 along with correlative data supporting Logic Gating mechanism of action Pipeline of NK and T cells for hard-to-treat cancers Potential best-in-class Logic-Gated cell therapy pipeline, initially targeting AML Lead program SENTI-202 First-in-Class Off-the-Shelf Logic-Gated Selective CD33 OR FLT3 NOT EMCN CAR NK Cell Therapy Investment from Leading Healthcare Institutional InvestorsPlatform technology can be utilized with both NK and T cell therapies Gene Circuits platform provides blockbuster opportunity to selectively and effectively target liquid and solid tumors while sparing healthy cells *CR: Complete Remission Scalable off-the-shelf manufacturing process streamlines treatment

Cancer Cells Healthy Cells Commercially approved CAR T cell therapies Recognize Single Antigen Target Single Antigen Target may be found on both cancer and healthy cells KILL KILL Biologics (ADC/TCE)* Logic Gates in Cell Therapy 4 Enable CAR-NK / CAR-T Cells to Address Broad Liquid and Solid Tumor Applications Non-Logic Gate Approaches Senti Bio’s Logic Gate Approach Cancer Cells Healthy Cells SENTI-202 and Other Undisclosed Programs KILL PROTECT (DO NOT KILL) OR Gate (aCAR) Kill if you see Antigens CD33 or FLT3 NOT Gate (iCAR) Do Not Kill if you see Antigen EMCN even if you see CD33 or FLT3 Recognizes Multiple Antigen Targets *ADC: antibody drug conjugate; TCE: T cell engagers

Acute Myeloid Leukemia (AML) Is an Aggressive Leukemia with Poor Prognosis • 20,800 newly diagnosed AML patients in US every year1 • ~60% patients experience relapse or death within 12 months2 AML Estimated Disease Burden Relapsed/Refractory AML Patient Outcome Effective Anti-AML Therapies Need To: • Current standard of care responses4,5 • CR rate ~15-25% • CR/CRh rate ~20-33% Median survival of 5.3 months3 5-year survival rate is 12.6%3 Target heterogenous clones / leukemia stem cells (LSCs)6 Selectively kill AML blasts and LSCs, and spare HSCs To achieve deep / MRD negative CR Leading to durable remissions / longer survival4,6 To support normal blood cell count recovery Leading to improved prognosis / longer survival7 1 2 CR: complete remission; CRh: complete remission with partial hematologic recovery; MRD: measurable residual disease; HSC: hematopoietic stem cell 1SEER 2024; 2Tenold Frontiers in Oncology 2021; 3Brandwein AJBR 2020; 4Dohner Blood 2022; 5USPI Idhifa, Xospata, Tibsovo; 6Zeijlemaker Leukemia 2019; 7Innes Blood 2018 5

SENTI-202: Intelligently Designed CAR-NK May Overcome Limitations of Current Therapies Against AML LSC: Leukemia stem cell; HSC: hematopoietic stem cell; HSPC: hematopoietic stem and progenitor cell 6 Bivalent CD33 and/or FLT3 activating CAR Calibrated release IL- 15 Endomucin inhibitory CAR to protect healthy cells Persistence, activation of CAR-NK and immune cells Healthy NK cells from selected adult donors FLT3 EMCN CD33 Healthy cell protection HSC aCAR iCAR Host immune cell SENTI-202 Cancer cell killing Blast cell CD33 LSC FLT3 SENTI-202 Gene Circuit Design • OR Logic Gate “Kills” leukemia blasts and LSCs via CD33 OR FLT3 activating CAR (aCAR) • CD33 and/or FLT3 expressed in ~95% of AML patients with CD33 being predominantly expressed on bulk blasts and FLT3 on LSCs • NOT Logic Gate “Protects” healthy HSC/HSPCs from ‘off-tumor, on-target’ effects • Protection of HSC/HSPCs via Endomucin (EMCN) inhibitory CAR (iCAR), even when they express CD33 and/or FLT3 • EMCN found predominantly on healthy HSC/HSPC surface, rarely on AML blasts • Calibrated release IL-15 “Enhances” SENTI-202 and host immune cell activity and persistence SENTI-202 is designed to selectively kill both AML blasts and LSCs while protecting healthy HSC/HSPCs using its novel CD33 OR FLT3 NOT EMCN Logic-Gated gene circuit

SENTI-202 Scalable Manufacturing Process Off-the-Shelf Allogeneic CAR-NK Lenti virus Outpatient use potential Isolate from selected donors Thaw and Infuse Off-the-Shelf Scalable Process NK Cells Selected Donor Engineer Cryopreserve and Store Expand SENTI-202 Gene Circuit delivered through single transduction step 1 2 3 4 1 Easy to thaw vials Final product harvested and cryopreserved NK cells isolated from peripheral blood of selected donors NK cells efficiently engineered with Gene Circuits High post- thaw potency Gene Circuit Engineered CAR- NK cells Patient 7

SENTI-202 Phase 1 Trial (SENTI-202-101) Design A multicenter, multinational, open-label study (NCT06325748) ECOG: European Cooperative Oncology Group; RP2D: recommended phase 2 dose; MTD: Maximum tolerated dose; DLT: dose limiting toxicity; G: Grade by CTCAE v5.0 and other applicable Grading systems; ELN: European LeukemiaNet; CyTOF: Cytometry by Time-of-Flight; BM: bone marrow 1Per WHO 2022 Classification; Key Eligibility Criteria Study Objectives • ≥18 and <75 years • ECOG 0-1 • R/R CD33 and/or FLT3 expressing heme malignancies • CD33+ by local assessment • R/R AML (1-3 prior Rx) • R/R MDS with increased blasts1 (1-2 prior Rx) • Must have received targeted agents if applicable mutations • Primary objective- safety and determination of MTD/RP2D • DLT definition includes: • ≥ G3 non-hematologic toxicities • Prolonged G4 neutropenia /thrombocytopenia not due to underlying disease • Other key objectives • Efficacy based on ELN 2022 criteria (AML) • MRD assessed per local standard of care • Pharmacokinetics (PK) • Pharmacodynamics (PDn) using CyTOF on serial BM samples Study Design Study Dosing “3+3” Dose finding followed by AML, MDS and other expansion cohorts at RP2D 2 Dose Levels and 2 schedules planned Opening dose cohort anticipated to be biologically active 8

SENTI-202 Phase 1 Trial (SENTI-202-101) Dosing Schema- Preliminary RP2D Identified 28Day -7 to -3 0 7 14 SENTI-202 Dose Schedule Efficacy Assessment Multiple cycles allowed to achieve optimal response Lymphodepletion Fludarabine/Cytarabine (Ara-C) 28Day -7 to -3 0 3 7 10 14 Dose SCHEDULE I SCHEDULE II SENTI-202 Dose Levels Opening Dose Cohort was Anticipated to be Biologically Active Schedule I Dose Level 1, N = 3 No DLTs Schedule I Dose Level 2, N = 3 No DLTs Identified as preliminary RP2D based on totality of clinical data Schedule II Dose Level 1, N = 3 No DLTs Multi-Dose Cycle Dose Level CAR+ NK Cells/Dose 1 1 x 109 2 1.5 x 109 9

preliminary RP2D Study Enrolled a High-Risk R/R AML Population with Multiple Baseline Adverse Characteristics Data from an open clinical database of an ongoing study as of 7 Apr 2025 Baseline Characteristics Dose Level 1 Dose Level 2 All Patients N = 9Schedule I N = 3 Schedule II N = 3 Schedule I N = 3 Age, yr, median (range) 64 (26,72) 41 (36, 67) 63 (51, 69) 63 (26, 72) Male, n (%) 1 (33) 2 (67) 3 (100) 6 (67) AML, n (%) 3 (100) 3 (100) 3 (100) 9 (100) Years from AML diagnosis, median (range) 2.84 (0.5, 6.2) 0.49 (0.3, 0.8) 0.94 (0.5, 1.0) 0.75 (0.3, 6.2) Adverse risk by ELN 2022, n (%) 2 (67) 2 (67) 3 (100) 7 (78) Baseline bone marrow blasts, %, median (range) 20 (15, 69) 30 (18, 31) 45 (10, 93) 30 (10, 93) Baseline platelet count < 50 x 109/L , n (%) 0 (0) 2 (67) 2 (67) 4 (44) • Median of <1 yr from diagnosis to trial entry across all patients and in preliminary RP2D cohort • Majority of patients (all in preliminary RP2D cohort) with adverse risk genetics by ELN 2022 criteria 10

preliminary RP2D Study Subjects had Received Multiple Prior Therapies Data from an open clinical database of an ongoing study as of 7 Apr 2025; * Primary refractory defined as failure to achieve cCR or cCR lasting <3 mo with front-line therapy • Median of 2 lines before study entry overall and in preliminary RP2D cohort • All patients with previous chemotherapy exposure including fludarabine and/or cytarabine (4 received both) and majority with previous venetoclax exposure Prior Therapy Dose Level 1 Dose Level 2 All Patients N = 9Schedule I N = 3 Schedule II N = 3 Schedule I N = 3 Number of prior lines, median (range) 1 (1,1) 2 (1, 2) 2 (2, 3) 2 (1,3) Chemotherapy, n(%) 3 (100) 3 (100) 3 (100) 9 (100) Fludarabine and/or Ara-C, n (%) 3 (100) 3 (100) 3 (100) 9 (100) Anthracycline, n (%) 3 (100) 2 (67) 3 (100) 8 (89) Venetoclax, n (%) 1 (33) 3 (100) 3 (100) 7 (78) Hypo-methylating Agents, n (%) 2 (67) 3 (100) 3 (100) 8 (89) FLT3/IDH2 targeted therapy, n (%) 1 (33) 1 (33) 1 (33) 3 (33) Bone marrow transplant, n (%) 1 (33) 0 (0) 1 (33) 2 (22) Primary refractory*, n (%) 1 (33) 2 (67) 2 (67) 5 (56) 11

preliminary RP2D Patients Received a Median of 2 Cycles of SENTI-202 Therapy Data from an open clinical database of an ongoing study as of 7 Apr 2025; cCR: composite CR includes CR, CRh, CRi • Median of 2 cycles across dose cohorts • Majority of patients discontinued SENTI-202 after achieving cCR with none discontinuing due to adverse event Exposure Dose Level 1 Dose Level 2 All Patients N = 9Schedule I N = 3 Schedule II N = 3 Schedule I N = 3 Number of SENTI-202 treatment cycles, median (range) 2 (1, 2) 2 (1, 2) 2 (1, 2) 2 (1, 2) Subjects continuing treatment as of data-cut, n (%) 0 2 (67) 0 2 (22) Subjects discontinuing treatment, n (%) 3 (100) 1 (33) 3 (100) 7 (78) Adverse Event 0 0 0 0 Achieved Optimal Response (cCR) 2 (67) 0 2 (67) 4 (44) Disease Progression/ Stable Disease 1 (33) 1 (33) 1 (33) 3 (33) 12